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KMID : 0370220220660050278
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Yakhak Hoeji
2022 Volume.66 No. 5 p.278 ~ p.282
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Evaluation of Anti-cancer Efficacy of Potassium Usnate using NIR Imaging of Orthotopic Breast Cancer Mouse Model
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Son Ye-Seon
Lee Chang-Wook
Yu In-Tag
Lee Mi-Jin
Kim Hangun
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Abstract
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Mouse cancer models are useful tools for evaluating in vivo tumor growth and metastasis, providing valuableinformation for preclinical testing. In this process, optical imaging enables the mouse models to easily identify the progressof disease in a non-invasive way. Here, we established an experimental bioimaging animal model of near-infrared (NIR)fluorescence by using a fluorescence-labeled organism bioimaging instrument (FOBI) and evaluated the anti-cancer effectof potassium usnate (KU) in an orthotopic breast cancer model. The cell viability assay revealed that KU had cytotoxicitywith half maximal inhibitory concentration of approximately 138.57, 167.69, and 144.17 μM in 4T1-Fluc-Neo/iRFP-Puro(4T1-iRFP), MDA-MB-231, and MCF-7 cells, respectively. The measurement of NIR fluorescence from the 4T1-iRFPcells in a microtube via FOBI exhibited a strong correlation between cell number and fluorescence intensity, and theminimal detection limit was 105 cells. Accordingly, NIR imaging was performed on the orthotopic breast cancer mousemodel by using FOBI, and regression of tumor progression through intraperitoneal KU administration was successfullymonitored. Our results demonstrated the establishment of NIR imaging in the orthotopic breast cancer animal model forevaluating the anti-cancer effect of KU.
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KEYWORD
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Breast cancer, 4T1 cell, Orthotopic breast cancer mouse model, Potassium usnate
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